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hiscript iii all  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript iii all
    Hiscript Iii All, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 2308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+iii+all/HiScript+III+All-in-one+RT+SuperMix+Perfect+for+qPCR/pm42905834-304-4-12
    Average 99 stars, based on 2308 article reviews
    hiscript iii all - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Physiological and biophysical evidence associates FeRbohB with rutin-mediated responses to PEG-simulated water deficit during common buckwheat germination
    Article Snippet: Article in Press BMC Plant Biology https://doi.org/10.1186/s12870-026-10079-7 qRT-PCR analysis Total RNA was extracted from plant tissues using the RNA Easy Fast Plant Tissue Kit (DP452, TIANGEN, Beijing, China). .. Subsequently, first-strand cDNA was synthesized from the extracted RNA using the HiScript III All-in-one RT SuperMix Perfect for qRT-PCR (R333-01, Vazyme, Nanjing, China) in accordance with the manufacturer’s instructions. .. The qRT-PCR assays were performed with the Taq Pro Universal SYBR qRT-PCR Master Mix (Q712, v20.1, Vazyme, Nanjing, China) following the manufacturer’s protocol.

    Article Title: Network toxicology, machine learning, single-cell analysis, and molecular docking reveal 6PPD/6PPD-Q-associated atrial fibrillation signatures.
    Article Snippet: Total RNA was extracted from both cultured cells and mouse cardiac tissues using the RNeasy Mini Kit (QIAGEN, 74004) following the manufacturer’s protocol. .. The cDNA was synthesized using the HiScript III All-in-one RT SuperMix Perfect Kit (Vazyme, R333). .. qRT-PCR was performed using ChamQ Blue Universal SYBR qPCR Master Mix (Q312; Vazyme).

    Article Title: CREBBP loss drives immune evasion by epigenetically silencing STING and MHC-I in small cell lung cancer.
    Article Snippet: Total RNA was extracted from tumor cells using TRIzol reagent (15596026CN, ThermoFisher). .. cDNA was synthesized using HiScript III All- in- one RT SuperMix (R333–01, Vazyme). .. Quantitative PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Q711–02, Vazyme).

    Article Title: Spatially Defined Latrunculin A-Eluting Intraocular Lenses Attenuate Posterior Capsule Opacification via Cytoskeletal Disruption.
    Article Snippet: Posterior capsule opacification (PCO), driven by the aberrant proliferation, migration, and fibrotic transformation of residual lens epithelial cells (LECs), remains the most common long-term complication of cataract surgery.. Given that intraocular lenses (IOLs) are precision optical implants, anti-PCO surface modifications should balance localized therapeutic delivery with preservation of a clear central optical zone.. Here, we developed spatially defined Latrunculin A (LatA)-eluting IOLs using a mask-assisted spin-coating

    Article Title: Phosphate rock drives greater arsenic accumulation in Pteris vittata than calcium carbonate by enhancing arsenic metabolism and reshaping rhizosphere microbiome.
    Article Snippet: Poorly-soluble phosphate rock (PR) and CaCO3 can enhance arsenic accumulation in calcicolous Ashyperaccumulator Pteris vittata, but their mechanisms under soil conditions remain unclear.. P. vittata was grown for two months in As-contaminated soil (100 mg kg–1) receiving 2.0 g kg–1 Ca as PR, CaCO3, or soluble CaCl2.. Plant nutrients, rhizosphere chemistry and bacterial communities, and As-metabolism genes were examined.

    Quantitative RT-PCR:

    Article Title: Physiological and biophysical evidence associates FeRbohB with rutin-mediated responses to PEG-simulated water deficit during common buckwheat germination
    Article Snippet: Article in Press BMC Plant Biology https://doi.org/10.1186/s12870-026-10079-7 qRT-PCR analysis Total RNA was extracted from plant tissues using the RNA Easy Fast Plant Tissue Kit (DP452, TIANGEN, Beijing, China). .. Subsequently, first-strand cDNA was synthesized from the extracted RNA using the HiScript III All-in-one RT SuperMix Perfect for qRT-PCR (R333-01, Vazyme, Nanjing, China) in accordance with the manufacturer’s instructions. .. The qRT-PCR assays were performed with the Taq Pro Universal SYBR qRT-PCR Master Mix (Q712, v20.1, Vazyme, Nanjing, China) following the manufacturer’s protocol.

    Reverse Transcription:

    Article Title: HSP27 Provides Stress-Adaptive Proteostasis Buffering in Glioblastoma and Is Therapeutically Vulnerable.
    Article Snippet: .. Reverse transcription was performed using the HiScript III All-in-one RT SuperMix kit (Vazyme, R333-01). .. mRNA abundance was determined by qPCR using the ChamQ SYBR Color qPCR Master Mix (Vazyme, Q421V23.1), with GAPDH used as the internal control.

    Article Title: The NEK8/ALYREF axis promotes cisplatin resistance in bladder cancer by stabilizing CDK18 mRNA to facilitate DNA damage repair.
    Article Snippet: Cisplatin-based chemotherapy remains a standard treatment for advanced bladder cancer (BCa), however, the development of chemoresistance substantially compromises its clinical efficacy and patient prognosis.. In this study, we investigated the functional role of NEK8 in cisplatin resistance in BCa and elucidated its underlying molecular mechanisms.. We found that NEK8 was significantly upregulated in cisplatin-resistant BCa and was associated with unfavorable prognosis.

    Article Title: Temporal Transcriptomic Profiling Reveals DECTIN-1–Mediated Immune Responses in Acanthamoeba Keratitis
    Article Snippet: .. The RNA was reverse transcribed into cDNA using the HiScript III All-in-One RT SuperMix (Vazyme, Nanjing, China), and qPCR with technical triplicates was performed using Taq Pro Universal SYBR qPCR Master Mix (Vazyme), following the manufacturer's guidelines. ..

    Real-time Polymerase Chain Reaction:

    Article Title: The NEK8/ALYREF axis promotes cisplatin resistance in bladder cancer by stabilizing CDK18 mRNA to facilitate DNA damage repair.
    Article Snippet: Cisplatin-based chemotherapy remains a standard treatment for advanced bladder cancer (BCa), however, the development of chemoresistance substantially compromises its clinical efficacy and patient prognosis.. In this study, we investigated the functional role of NEK8 in cisplatin resistance in BCa and elucidated its underlying molecular mechanisms.. We found that NEK8 was significantly upregulated in cisplatin-resistant BCa and was associated with unfavorable prognosis.

    Article Title: Temporal Transcriptomic Profiling Reveals DECTIN-1–Mediated Immune Responses in Acanthamoeba Keratitis
    Article Snippet: .. The RNA was reverse transcribed into cDNA using the HiScript III All-in-One RT SuperMix (Vazyme, Nanjing, China), and qPCR with technical triplicates was performed using Taq Pro Universal SYBR qPCR Master Mix (Vazyme), following the manufacturer's guidelines. ..

    Article Title: Spatially Defined Latrunculin A-Eluting Intraocular Lenses Attenuate Posterior Capsule Opacification via Cytoskeletal Disruption.
    Article Snippet: Posterior capsule opacification (PCO), driven by the aberrant proliferation, migration, and fibrotic transformation of residual lens epithelial cells (LECs), remains the most common long-term complication of cataract surgery.. Given that intraocular lenses (IOLs) are precision optical implants, anti-PCO surface modifications should balance localized therapeutic delivery with preservation of a clear central optical zone.. Here, we developed spatially defined Latrunculin A (LatA)-eluting IOLs using a mask-assisted spin-coating



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